R5277
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RT-PCR Protocols
| ISBN-10: | 0-89603-875-0 |
| ISBN-13: | 978-0-89603-875-2 |
Description
| General description | A panel of molecular biologists and clinical researchers describe their novel, useful, and interesting RT-PCR applications. One will find reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For someone seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering |
Properties
| publication info | J. O'Connell, ed., Humana Press, 2002, 380 pp., hard cover |
Table Of Contents
| Table of Contents | Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA The Basics of RT-PCR: Some Practical Considerations Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples Detection of mRNA Expression and Alternative Splicing in a Single Cell Nested RT-PCR: Sensitivity Controls are Essential to Determine the Biological Significance of Detected mRNA Part III. Quantitative RT-PCR Quantitative RT-PCR: A Review of Current Methodologies Rapid Development of a Quantitative-Competitive (QC) RT-PCR Assay Using a Composite Primer Approach Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Products Time-Resolved Fluorometric Detection of Cytokine mRNAs Amplified by RT-PCR Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytes Part IV. Detection and Analysis of RNA Viruses Detection and Quantification of the Hepatitis C Viral Genome Semi-Quantitative Detection of Hepatitis C Virus RNA by "Real-Time" RT-PCR RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus Part V. In Situ Localization of mRNA Expression In Situ Immuno-PCR: A Newly Developed Method for Highly Sensitive Antigen Detection In Situ RT-PCR from Laser Capture Microdissected Samples Mycobacterium Paratuberculosis Detected by Nested PCR in Intestinal Granulomas Isolated by Laser Capture Microdissection in Cases of Crohn's Disease RT-PCR-Based Approaches to Generate Probes for mRNA Detection by In Situ Hybridization Part VI. Differential mRNA Expression Amplified RNA for Gene Array Hybridizations Semiquantitative Determination of Differential Gene Expression in Primary Tumors and Matched Metastases by RT-PCR: Comparison with Other Methods Part VII. Genetic Analysis Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay Part VIII. RT-PCR in Immunology Detection of Clonally Expanded T Cells by RT-PCR-SSCP and Nucleotide Sequencing of T Cell Receptor b-CDR3 Regions Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B Cells Generation of Murine scFv Intrabodies from B Cell Hybridomas Quantitation of mRNA Levels by RT-PCR in Cells Purified by FACS: Application to Peripheral Cannabinoid Receptors in Leukocyte Subsets Part IX. RT-PCR in Antisense Technology Detection of Antisense RNA Transcripts by Antisense RT-PCR Part X. RT-PCR in cDNA Cloning RT-PCR in cDNA Library Construction An RT-PCR-Based Protocol for the Rapid Generation of Large, Representative cDNA Libraries Suitable for Expression Screening Index |
